Coding
H/E-rev
Part:BBa_K1616005:Design
Designed by: Johanna Chesnel Group: iGEM15_IONIS_Paris (2015-09-17)
Holin/endolysin reversed - RBS and double T7 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 306
Illegal AgeI site found at 376 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
Source
BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (double terminator T7 reversed).
References
Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4